Colonnes en phase inverse pour peptides
Columns
Descriptif
La chromatographie en phase inverse utilisant des réactifs d’appariement d’ions tels que le TFA et l’acide formique sépare de manière très résolutive les mélanges peptidiques complexes (par exemple, les digestions tryptiques de protéines ou les longues séquences de peptides synthétiques) dont les séquences peuvent différer d’un seul acide aminé. L’hydrophobicité du peptide détermine l’ordre d’élution, les peptides les moins hydrophobes éluant en premier. Des facteurs tels que la composition des particules (silice ou hybrides), la taille des pores (130 Å ou 300 Å), et la densité du ligand, ainsi que les conditions de séparation (par exemple, durée du gradient, température de la colonne, débit) jouent tous un rôle important dans l’obtention de la séparation attendue. Comme pour les autres séparations, lorsque l’on utilise des particules de plus petite taille pour les modes UHPLC et UPLC, les colonnes offrent une meilleure résolution
- Laboratoires - appareils et instruments
- Colonnes Phase
- Peptides Colonnes
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