Sélectivité optimale quel que soit le pH pour des séparations plus efficaces Jusqu’ici, les limites des matériaux des phases stationnaires impactaient la vitesse, la résolution, la capacité de charge ainsi que la stabilité face au pH et à la température lors de séparations HPLC. Les colonnes HPLC XTerra conservent les avantages de la silice sans perte de vitesse, de résolution, de capacité de charge ni de stabilité au pH ou à la température. Les colonnes XTerra, qui utilisent la technologie HPT (Hybrid Particle Technology) de première génération, ont dépassé ces limites et permis à des scientifiques comme vous de réaliser le plein potentiel des séparations analytiques et préparatives. Menez à bien vos séparations les plus complexes grâce aux chimies de colonnes XTerra, telles que les colonnes C18, C8 et Phényle, qui offrent une large gamme d’options de sélectivité.
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Durée de vie accrue des colonnes à pH > 8,0 Séparation efficace des composés basiques à pH élevé Réduction des silanols de surface pour des pics exceptionnellement nets et à haute efficacité pour les composés basiques Performances analytiques dans les dimensions préparatives avec une stabilité de colonne maximale.
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High pore volume HPLC particles do not possess the mechanical stability necessary to withstand the high pressures inherent in UPLC® separations. Waters material scientists addressed this challenge by developing a silica particle designed for high mechanical stability with the appropriate morphology to provide long UPLC column lifetimes and high UPLC efficiencies at high pressures. The 1.8 µm High Strength Silica (HSS) particle is the first and only 100% silica particle designed, tested and intended for use in applications up to 15,000 psi (1034 bar). HSS particle technology is also available in HPLC particle sizes (3.5 and 5 µm) in the HSS family of HPLC columns, enabling seamless transfer between HPLC and UPLC technology platforms.
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The 1.7 µm Charged Surface Hybrid (CSH™) particle is Waters third generation hybrid particle technology. Based on Waters Ethylene Bridged Hybrid (BEH) particle technology, CSH particles incorporate a low level surface charge that improves sample loadability and peak asymmetry in low-ionic-strength mobile phases, while maintaining the mechanical and chemical stability inherent in the BEH particle technology. The advantages of CSH Technology UPLC chromatography columns include: Superior peak shape for basic compounds Increased loading capacity Rapid column equilibration after changing mobile-phase pH Improved batch-to-batch reproducibility Exceptional stability at low and high pH Click here for Waters new CSH130 columns for peptide separations. CSH particle technology is also available in HPLC particle sizes (3.5 and 5 µm) in the XSelect™ family of HPLC columns, enabling seamless transfer between HPLC and UPLC® technology platforms.
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The 1.7 µm Ethylene Bridged Hybrid [BEH] particle is one of the key enablers behind UPLC Technology. It is available in three different pore sizes [130Å, 200Å, and 300Å] and several bonded phases for reversed-phase and hydrophilic interaction chromatography and is applicable from small molecule to large biopharmaceutical analysis. Due to the intrinsic chemical stability of hybrid particle technology, a wider usable pH range [pH 1-12] can be employed, enabling a versatile and robust separation technology for method development. BEH particle technology is available in HPLC particle sizes [2.5, 3.5, 5 and 10 µm] in the XBridge family of HPLC columns, enabling seamless transfer between HPLC and UPLC technology platforms. ACQUITY UPLC BEH C18 and C8 Columns Providing unprecedented levels of peak asymmetry, efficiency, and chemical stability, the 1.7 µm ACQUITY UPLC BEH C18 and C8 Columns are versatile, high-performance separation columns suitable
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